Curriculum Vitae

Christina L. Graves

Department of Oral Biology, University of Florida

Gainesville, FL 32608

352-276-8135 (phone)

cgraves@dental.ufl.edu

EDUCATION

University of Florida, Gainesville, FL,  08/2011 – present
College of Medicine, Interdisciplinary Biomedical Sciences Program (IDP)

Georgia Institute of Technology, Atlanta, GA,  08/2006 –05/2011
Bachelor of Science, Major: Biology, Honors Program Member

RESEARCH EXPERIENCE

University of Florida
Mentor: Shannon M. Wallet, PhD, Department of Periodontology, 03/2011 – present

Currently investigating the role of intestinal epithelial cells in the onset and outcome of systemic autoimmune disease, particularly that of type 1 diabetes (T1D). Focus on TLR signaling of intestinal epithelial cells. Dissertation: “Aberrant TLR signaling in the GI tract contributing to a break in self-tolerance in T1D”

Georgia Institute of Technology
Mentor: Eric Gaucher, PhD, School of Biology, 08/2010 – 08/2011

Investigated the transcriptional and translational features and limitations of human uricase (UOX). Became proficient in PCR techniques, gel electrophoresis and extraction, primer design, bacterial and human cell line growth, transfection, protein assays, and other common wet lab techniques. Received a SURE Fellowship from Emory University for Summer 2011. Research resulted in publication (Kratzer et al. 2014).

Mars Desert Research Station (MDRS),  08/2010 – 04/2011

2010-2011 Georgia Tech Crew 101 Chief Scientist

A two week-long research simulation at the Utah MDRS. On site, I conducted extremophile research, small molecule research, as well as investigated habitable zones by soil analysis.

International Genetically Engineered Machine Competition (iGEM),  08/2010 – 11/2010

Georgia Tech Team Member

Investigated the thermogenic capabilities of alternative oxidase (AOX) in bacteria in response to a cold shock. Analytical techniques include bacterial cell plating and culture, PCR, purification and ligation techniques, primer design, sequencing, synthetic bacterial design and “construction” using the BioBricks format, gel eletrophoresis, and in vivo heat characterization. The team won the Silver Prize at the international competition held at Massachusetts Institute of Technology.

Mentor: Nick V. Hud, PhD, Dept. of Chemistry & Biochemistry, 12/2006 – 05/2010

Conducted research on plausible prebiotic chemical pathways using formamide and mineral catalysts. Analytical techniques employed include GC-MS, HPLC, UV-VIS, NMR. Assisted in lab protocol development for local high schools through the GIFT program. Received Presidential Undergraduate Research Award (PURA). Research resulted in publication (Anumukonda et al 2011).

RESEARCH SUPPORT

Ruth L. Kirchstein NRSA Individual Predoctoral Fellowship F31 (Graves/Wallet)                07/2015 – 07/2017

NIH/NIDDK, “Gastrointestinal Epithelial Cell Immune Regulation of Autoimmunity in T1D.”
1F31DK104492-01A1 NIH/NIDDK (Role: PI) University of Florida, Gainesville, FL

NIH Predoctoral Grant Graduate Trainee                                                                             07/2012 – 07/2015
5T90 DE-021990-02, University of Florida, Gainesville, FL

PUBLICATIONS

Peer-Reviewed Scientific Publications

Jones, MK, Grau, KR, Costantini, V, Kolawole, AO, de Graaf, M, Freiden, P, Graves, CL, Koopmans, M, Wallet, SM, Tibbetts, SA, Schultz-Cherry, S, Wobus, CE, Vinje, J, Karst, SM. Human Norovirus Culture in B Cells. Nature Protocols. 2015 Dec;10(12):1939-47. doi: 10.1038/nprot.2015.121. link. Featured on Science Daily.

Delitto, D, Perez C, Han, S, Gonzalo, DH, Pham, K, Knowlton, AE, Graves, CL, Behrns, KE, Moldawer, LL, Thomas RM, Liu C, George TJ, Trevino, JG, Wallet, SM, Hughes, SJ. Downstream mediators of the intratumoral interferon response suppress antitumor immunity, induce gemcitabine resistance and associate with poor survival in pancreatic cancer. Cancer Immunol Immunother. 2015 Dec;64(12):1553-63. doi: 10.1007/s00262-015-1760-y. Epub 2015 Sep 30. link.

Valladeres, R, Graves, CL, Wright, K, Gardner, C, Wallet, SM, Lorca, GL, Gonzalez, CF. H2O2Production Rate in Lactobacillus johnsonii is Modulated Via the Interplay of a Heterodimeric Flacin Oxidoreductase with a Soluble 28 Kd PAS Domain Containing Protein. Front. Microbiol. 2015 July 14. July 14;6:716. PMID:26236298. link.

Jones, MK, Watanbe, M, Zhu, S, Graves, CL, Keyes, LR, Grau, KR, Gonzalez-Hernandez, MB, Iovine, NM, Wobus, CE, Vinje, J, Tibbetts, SA, Wallet, SM, Karst, SM. Enteric bacteria promote human and mouse norovirus infection of B cells. Science. 2014 Nov. 7. PMID: 25378626. linkNational Geographic feature by Carl Zimmer (link).  Featured on TWiV (link).

Graves, CL, Harden, SW, Lapato, M, Nelson, M, Amador, M, Sorenson, S, Frazier, CJ, and Wallet, SM. A Method for High Purity Intestinal Epithelial Cell From Adult Human and Murine Tissues for the Investigation of Innate Immune Function. J. Imm. Methods. Epub 2014 Sep 2. PMID: 25193428. link  *JIM Highlighted Article

Kratzer, JT, Lanaspa, MA, Murphy, MN, Cicerchi, C, Graves, CL, Tipton, PA, Ortlund, EA, and Johnson, RJ. Evolutionary History and Metabolic Insights of Ancient Mammalian Uricases. Proc Natl Acad Sci. 2014. March 11:111(10):3763-8 PMID: 24550457. linkNational Geographic Feature by Ed Yong (link).

Anumukonda, LN, Young, A, Lynn, DG, Buckley, R, Warrayat, A, Graves, CL, Bean, HD, and Hud, NV. Adenine Synthesis in a Model Prebiotic Reaction: Connecting Origin of Life Chemistry with Biology. J. Chem. Educ. 2011 Dec 1:88(12):1698-1701 PMID: 22075932. link

Submitted and In press

Graves, CL, Lapato, M, Li, J, Knowlton, A, Wallet, MA, Wallet SM. Altered mucosal homeostasis and intestinal epithelial cell dysfunction in human type 1 diabetes. Submitted.

Non-peer Reviewed Publications

Graves, CL. Retraction of Paper “Confirming” Narcolepsy as an Autoimmune Disease. AutoimmunePatient.com Blog. 30 July 2014.
Graves, CL. Paleo Narco: Applying “Paleo” Principles to Narcolepsy. Paleo Movement Magazine. 17 Oct. 2014.
Graves, CL. Recent Science Headlines Relevant to Ancestral Health. Paleo Movement Magazine. 6 June 2014.
Graves, CL. 19 Gluten Cross-Reactive Foods – Busted Myth? Paleo Movement Magazine. 9 May. 2014.
Graves, CL. Interesting Science Updates. Paleo Movement Magazine. 14 Feb. 2014.
Graves, CL. 2013 Paleo Year in Review. Paleo Movement Magazine. 3 Jan. 2014.
Graves, CL. Important Ancestral Health Advances of 2013. Paleo Movement Magazine. 3 Jan. 2014.
Graves, CL. Interview with Joe Salama about the Ancestral Food Summit. Paleo Movement Magazine. 6 Dec. 2013.
Graves, CL. 2013 Ancestral Health Symposium Conference Recap. Paleo Movement Magazine. 22 Aug. 2013.
Graves, CL, and Gina Singh. Advocating for Your Narcoleptic Child: Tips for Success. The Network: Fourth Quarter. 2012.
Graves, CL. Sleepy Shots: Flu Vaccine and Childhood Narcolepsy. The Network: Fourth Quarter. 2012.
Graves, CL. Question and Answer Session with a University Learning Specialist. The Network. Second Quarter. 2012.
Graves, CL. and Ashley Starr. How to Deal with College like a Narcolepsy Advocate All-Star. The   Network. Second Quarter. 2012.

POSTER AND ORAL PRESENTATIONS

Graves, CL, Lapato, M, Li, J, Shapiro, M, Wallet MA, Glover, S, Wallet, SM. TLR5-Mediated Intestinal Epithelial Cell Dysfunction I Type 1 Diabetes. Graduate Student Research Day. University of Florida. Gainesvile, FL. October, 2015.

Graves, CL, Lapato, M, Li, J, Knowlton, AK, Wallet, MA, Wallet, SM. Dysregulated Intestinal Innate Immune Function in Human Type 1 Diabetes. W44.26. International Congress of Mucosal Immunology (ICMI) 2015. Berlin, Germany. 14-18 July 2015. *published abstract and poster presentation

Wallet, SM, Hulme, MA, Nelson, MR, Graves, CL, Sorenson, HL, Brusko, T. Altered Gastrointestinal Environment and Immune Cellular Plasticity During Disease Progression of Type 1 Diabetes. Abstract 91; 65.14 (MUC2P.931). Immunology 2015. New Orleans, LA. 9 May 2015.

Graves, CL, Lapato M, Wallet, SM. Intestinal Epithelial Cell Mediated Miscommunication in Type 1 Diabetes: A Tipping Point for Disease? Abstract pg. 91; 65.16 (MUC2P.933). Immunology New Orleans, LA. 9 May 2015.

Hulme, MA, Nelson, MR, Graves, CL, Amador, BA, Brusko, T., Wallet, SM. Altered Gastrointestinal Envrionment and Immune Cellular Plasticity During Disease Progression of Type 1 Diabetes. Immunology of Diabetes Society (IDS) 14th International Congress. Munich Gemany. 12-16 Apr 2015

Harden, SW, Graves, CL, Wallet, SM, Frazier, CJ. Oxytocin Receptor Activation Depolarizes Spiny Hilar Interneurons and Induces GABA Release in the Dentate Gyrus of the Rat Hippocampus. UF Brain Awareness Week Symposium. 20 March 2015.

Duque Rosas, S, Bainbridge, B, Knowlton, A, Graves, C, Wallet, SM, Davey, M. L-Arginine Affects Adherence of Pyphoramonas gingivalis to Oral Epithelial Cells. Abstract number S1585. International Association for Dental Research (IADR) General Session. Boston, MA. 12 March 2015.

Graves, CL, Lapato M, Wallet, MA, Wallet, SM. Dysregulated Gastrointestinal Innate Immune Function in Human Type 1 Diabetes. Abstract number 16.College of Dentistry Spring Synergy – Celebration of Research Day. Gainesville, FL. 10 Apr 2015.  *2nd place Excellence in Research Award

Graves, CL, Lapato M, Wallet, MA, Wallet, SM. Environment-Host Miscommunication at the Intestinal Epithelial Interface in Type-1 Diabetes: ­A Tipping Point for Disease? JDRF nPOD Annual Scientific Symposium. St. Petersburg, FL. February 2015.

Graves, CL. Gluten Free PWN: Improving Narcolepsy Symptoms Using Dietary Strategies. Narcolepsy Network 29th Annual Conference. Denver, CO. 19 Oct 2014. *invited oral presentation.

Graves, CL, Lapato, M, Wallet, SM. Intestinal Epithelial Cell Dysfunction in Type 1 Diabetes. University of Colorado, Anschutz Medical Campus. Denver, CO. 20 Oct 2014.

Graves, CL, LaPato, M, Wallet, SM. Environment-Host Miscommunication at the Intestinal Epithelial Interface in Type 1 Diabetes- A Tipping Point for Disease? Graduate Student Research Day. Abstract pg. 39. University of Florida. Gainesville, FL. Oct 2014.

Graves, CL, LaPato, M, Wallet, SM. Environment-Host Miscommunication at the Intestinal Epithelial Interface in Type 1 Diabetes- A Tipping Point for Disease? Joint meeting of the Society for Leukocyte Biology and the International Endotoxin and Innate Immune Society. JLB Supplement 2014. Page S38 Abstract #71. Salt Lake City, Utah. Oct 2014.

Graves, CL and Wallet, SM. Intestinal Epithelial Cell Dysfunction in Type 1 Diabetes. College of Dentistry Spring Synergy – Celebration of Research Day. Gainesville, FL. April 2014. *oral presentation

Graves, CL and Wallet, SM. Intestinal Epithelial Cell Dysfunction in Type 1 Diabetes. UF IDP Immunology and Microbiology Concentration Seminar Series. Gainesville, FL. April 2014. *oral presentation

Graves, CL and Wallet, SM. Intestinal Epithelial Cell Dysfunction in Type 1 Diabetes (#308). University of Florida College of Medicine Celebration of Research. March 2014. *poster presentation

Graves, CL and Wallet, SM. University of Florida Three Minute Thesis Preliminary Heat Competition. Gainesville, FL. March 2014. *oral presentation

Graves, CL and Wallet, SM. Intestinal Epithelial Cell Dysfunction in Type 1 Diabetes. 2014 MSI Medical Guild Internal Competition. Gainesville, FL. March 2014. *oral presentation

Graves, CL and Wallet, SM. Intestinal Epithelial Cell Dysfuction in Type 1 Diabetes. JDRF nPOD Annual Scientific Symposium. Atlantic Beach, FL. February 2014. *invited oral presentation and poster

Graves, CL and Wallet, SM. Intestinal Epithelial Cell Immune Dysregulation in Type 1 Diabetes. UF Graduate Student Research Day. Gainesville, FL. October 2013. *poster presentation

Graves, CL and Wallet, SM. Intestinal Epithelial Cell Dysfuction in Type 1 Diabetes. Society for Leukocyte Biology. Newport, RI. October 2013. *poster presentation

Graves, CL and Wallet, SM. I Love Your Guts: Intestinal Epithelial Cells and Type 1 Diabetes. UF Whitney Laboratory Biology at the Beach Symposium. St. Augustine, FL. June 2013. *oral presentation

Graves, CL and Wallet, SM. Intestinal Epithelial Cells in T1D. UF College of Dentistry Research Day. Gainesville, FL. May 2013. *poster presentation.

Graves, CL and Gaucher, EA. Translational Limitations and Features of Human Uricase. Emory University SURE Research Conference. Atlanta, GA. August 2011. *poster presentation

Graves, CL and Gaucher, EA. Translational Limitations and Features of Human Uricase & GT iGEM: Using Synthetic Biology to Create a Bacterial Heater. University of Miami ACC Meeting of the Minds Conference. Coral Gables, FL. April 2011. *poster presentation

Graves, CL and Gaucher, EA. The Mars Desert Research Station: Georgia Tech Crew 101. Georgia Tech Honors Program Spring Expo. Atlanta, GA. April 2011. *poster presentation

Graves, CL and Gaucher, EA. Translational Limitations and Features of Human Uricase. Georgia Institute of Technology Undergraduate Research Spring Symposium. Atlanta, GA. April 2011. *poster presentation

iGEM 2010 Team (PI: Gaucher, EA). Inducing a Thermogenic Response to Cold Shock in Escherichia coli. International Genetically Engineered Machine Competition (iGEM). URK Pecha Kucha. Emory University, Atlanta, GA. December. 2010. *oral presentation

ACADEMIC AND PROFESSIONAL HONORS

University of Florida

1F31DK104492-01A1 NIH/NIDDK NRSA Individual Predoctoral Fellowship, 2015 – present
5T90 DE-021990-02 NIH Predoctoral Grant Graduate Trainee, 2012 – present
UF IDP Fellowship Scholarship,  2015
UF Graduate Student Council International Travel Award,  2015
Excellent in Research Award, College of Dentistry Research Day – 2nd place, 2015
Interdisciplinary Program in Biomedical Sciences Student Travel Award,  2014
2014 SLB IEIIS Meeting Travel Award, 2014
MSI Medical Guild Internal Competition – runner up for 2nd place, 2014
MSI Medical Guild Advancement to Candidacy Award – Nominee,  2014
Three Minute Thesis (3MT) Competition – finalist,  2014

Georgia Institute of Technology

Jimmy Rane Foundation Scholar,  2006 – 2011
Robert C. Byrd Scholar, 2006 – 2011
Georgia Institute of Technology Dean’s List, 2007, 2010, 2011
John Staton Scholar, 2007, 2008, 2010
Presidential Undergraduate Research Award (PURA), Hud Lab, 2008
Silver Medal, MIT International iGEM Competition, 2010
Roy & Zou Feagin Scholarship Award, 2010
Georgia Tech School of Biology Representative, ACC Meeting of the Minds, 2010
SURE Program Fellowship – Emory University, 2011
School of Biology, Travel Grant, 2011

TEACHING, MENTORSHIP AND OUTREACH

UF SSTP Program Course Leader and Lead Instructor, 2015
IDP Recruitment Volunteer, 2015
Three Minute Thesis (3MT) Finalists Panel, 2015
UF IDP Peer Mentorship, 2012-2014
UF IDP 1st Year Student Tutor, 2014
UF GMS 6001 Computer-Based Learning Course Developer, 2012
Undergraduate Mentorship (Melissa LaPato), 2013 – current
UF Disability Resource Center “Dining With Disabilities,” Invited Speaker, 2013

Additional Seminars, Symposiums, Professional Training and Conferences Attendance

Mucosal Immunology School: Pillars in Immunology, ICMI2015, 2015
Center for Inflammation and Mucosal Immunology (CIMI) Mini Symposium, 2015
Advanced FlowJo Training, University of Florida, 2014
Oral Biology Departmental Retreat, 2014
Street Smarts of Science, SLB Annual Conference,  2013, 2014
Grant Writing Workshop, SLB Annual Conference, 2013, 2014
Narcolepsy Network Member Conference, 2011, 2012, 2014
UF T Cell Retreat: Cellular Metabolism and Autoimmunity, 2013
Ancestral Health Symposium, Atlanta, GA., 2013
Biology at the Beach, St. Augustine, FL , 2013
Diabetes Lab Mixer and Poster Presentation, 2013

Editorial board

Journal of Evolution and Health (JEVO) – Associate Editor, 2013 – present

Professional Memberships and Community Activities

American Diabetes Association (ADA)  – Member, 2014 – present
American Association for the Advancement of Science (AAAS) – Member, 2014 – present
American Association of Immunologists (AAI) – Member, 2014 – present
UF Center for Inflammation and Mucosal Immunology (CIMI) – Member 2012 – present
Society of Leukocyte Biology (SLB) – Member, 2013 – present
American Chemical Society (ACS) – Member, 2010 – present
Narcolepsy Network – Member, Writer, 2011 – present
Ancestral Health Society – Member, 2012 – present
Society for Mucosal Immunology – Member, 2012 – present
ThePaleoMovement.com – Scientific Writer, 2013 – present
Alpha Chi Sigma Professional Chemistry Fraternity – Member, 2007 – present
American Society for Molecular Biology (ASM) – Member, 2010 – 2012
Active Minds at Georgia Tech –  President,  2006 – 2011
American Free Thought Podcast (#91)- Guest speaker, 2010
Gwinnett School of Mathematics, Science and TechnologyScience Fair – Judge, 2009